Generation of hydrogen peroxide by cerebral-cortex synaptosomes. Stimulation by ionomycin and plasma-membrane depolarization
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چکیده
منابع مشابه
Effect of Paraoxon on GABA Uptake by Rat Cerebral Cortex Synaptosomes
Background: It has been suggested that organophosphates may inhibit gamma-aminobutyric acid (GABA) metabolism in synaptosomal preparations. In the present investigation, we have assessed the interaction between paraoxon and the GABA system at synaptic level. Methods: Synaptosomes were prepared from male Wistar rats (200-250 g). Cerebral cortex was dissected and homogenized, then centrifuged at ...
متن کاملeffect of paraoxon on gaba uptake by rat cerebral cortex synaptosomes
background: it has been suggested that organophosphates may inhibit gamma-aminobutyric acid (gaba) metabolism in synaptosomal preparations. in the present investigation, we have assessed the interaction between paraoxon and the gaba system at synaptic level. methods: synaptosomes were prepared from male wistar rats (200-250 g). cerebral cortex was dissected and homogenized, then centrifuged a...
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Indirect evidence has suggested that K-Cl cotransport in human and sheep erythrocytes is activated physiologically by a serine-threonine phosphatase. It is activated experimentally by H2O2and by staurosporine, a kinase inhibitor. Activation by H2O2and staurosporine is inhibited by serine-threonine phosphatase inhibitors, suggesting that the activators stimulate the phosphatase. The present stud...
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Receptor-stimulated generation of reactive oxygen species (ROS) has been shown to regulate signal transduction, and previous studies have suggested that T cell receptor (TCR) signals may involve or be sensitive to ROS. In this study, we have shown for the first time that TCR cross-linking induced rapid (within 15 min) generation of both hydrogen peroxide and superoxide anion, as defined with ox...
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The thyroid plasma membrane contains a Ca2(+)-regulated NADPH-dependent H2O2 generating system which provides H2O2 for the thyroid peroxidase-catalyzed biosynthesis of thyroid hormones. The plasma membrane fraction contains a Ca2(+)-independent cytochrome c reductase activity which is not inhibited by superoxide dismutase. But it is not known whether H2O2 is produced directly from molecular oxy...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1989
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1989.tb14670.x